PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 1

Entry No. - 1   [TOP]
PolysacDB ID2025
Carbohydrate NameCore oligosaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobePseudomonas aeruginosa   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core region of LPS is divided into the outer core and inner core. The inner core region consists of 2-keto-3-deoxyoctonic acid (KDO), heptose, phosphate, and probably ethanolamine. The outer core region commonly contains D-glucose, L-rhamnose, D-galactosamine, and L-alanine. The O-antigens consist of a trisaccharide repeating unit which consists of two uronic acid derivatives and one N-acetyl fucosamine residue
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat killed Whole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 360.7
Antibody type and classIgG2b
Assay SystemELISA, flow cytometry, immunoblotting and immunogold electronmicroscopy
Cross-reactivityThe Mab cross-reacted with some of the other Pseudomonas spp such as P . Acidovorans, P.fluorescens, P. mendocina, P . pseudoalkaligenes, P. putida and P . stutzeri., but not with P. cepacia or Xanthomonas (Pseudomonas) maltophilia
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab can be used for an immunological assay for detection of P. aeruginosa in respiratory secretions of cystic fibrosis patients
Curator IDAA + AS
Date of Curation20-12-2010
References1588587


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