PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 3

Entry No. - 1   [TOP]
PolysacDB ID1374
Carbohydrate NameUnknown carbohydrate   (Drugpedia)
Carbohydrate ClassGlycoprotein
MicrobeCoccidioides immitis   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesCoccidiodin along with spherulin
Carrier NameNil
Conjugation MethodNil
AntibodiesMab
Antibody type and classIgG1
Assay SystemELISA, immunoblotting and 2d-immunoelectrophoresis
Cross-reactivityThis antibody was specific to Coccidiodes and did not cross-react with histoplasmin and blastocyanin
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis MAb would be useful as a probe for detecting circulating antigen in patients with coccidioidal disease and to assess the effect of passively transferred Mab on the pathogenesis of experimental murine coccidioidomycosis
Curator IDAA + AS
Date of Curation02-07-2010
ReferencesPMC313225


Entry No. - 2   [TOP]
PolysacDB ID1634
Carbohydrate NamePhenolic glycolipid antigen   (Drugpedia)
Carbohydrate ClassGlycolipid
MicrobeMycobacterium leprae   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt consisting of terminal 3,6-di-O-methyl glucose linked β1-->4 to 2,3-di-O-methyl rhamnose, which in turn is linked α1-->2 to 3-O-methyl rhamnose
BCSDB Structure114263
Proposed functionsIt has been proposed to protect pathogenic mycobacteria such as Mycobacterium lepraemurium from the host bactericidal activities in phagocytic cells
Antigenic Nature used to produce antibodiesPartially purified phenolic glycolipid from M. leprae
Carrier NameNil
Conjugation MethodNil
AntibodiesMab
Antibody type and classIgM
Assay SystemELISA, TLC autoradiography and immunofluorescence
Cross-reactivityThis Mab was specific to M. leprae glycolipid
Proposed epitopesTerminal 3,6-di-O-methyl glucose residues were proposed to be a part of the epitope
IEDB Epitope76895
Proposed UtilityThis Mab may provide more information on the structure of phenolic glycolipid of M. leprae and the localization of this antigen can be performed within infected lesions by immunofluorescence
Curator IDAA + AS
Date of Curation26-09-2010
ReferencesPMC263407


Entry No. - 3   [TOP]
PolysacDB ID1778
Carbohydrate NameUnknown carbohydrate   (Drugpedia)
Carbohydrate ClassGlycoprotein
MicrobeMycoplasma mycoides   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesM. mycoides subsp. Mycoides integral membrane protein
Carrier NameNil
Conjugation MethodNil
AntibodiesMab
Antibody type and classIgM
Assay SystemELISA, immunoblotting
Cross-reactivityN/A
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab had invivo growth inhibitory activity
Curator IDAA + AS
Date of Curation29-10-2010
ReferencesPMC170441


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