1SAY Oxidoreductase date Jun 05, 1998
title L-Alanine Dehydrogenase Complexed With Pyruvate
authors P.J.Baker, Y.Sawa, H.Shibata, S.E.Sedelnikova, D.W.Rice
compound source
Molecule: L-Alanine Dehydrogenase
Chain: A
Ec: 1.4.1.1
Engineered: Yes
Biological_unit: Homohexamer
Organism_scientific: Phormidium Lapideum
Expression_system: Escherichia Coli
Expression_system_strain: Mv1184
Expression_system_vector: Puc18
Expression_system_plasmid: Pnad1
Expression_system_gene: Aladh
Other_details: Matsue Hot Springs
symmetry Space Group: H 3 2
R_factor 0.18
crystal
cell
length a length b length c angle alpha angle beta angle gamma
122.400 122.400 183.000 90.00 90.00 120.00
method X-Ray Diffractionresolution 2.1 Å
ligand PYR enzyme 1.4.1.1. Oxidoreductase E.C.1.4.1.1 BRENDA
note 1SAY is a representative structure
Gene
Ontology
ChainFunctionProcessComponent
A
  • alanine dehydrogenase activi...
  • oxidoreductase activity
  • electron transport

  • Primary referenceAnalysis of the structure and substrate binding of Phormidium lapideum alanine dehydrogenase., Baker PJ, Sawa Y, Shibata H, Sedelnikova SE, Rice DW, Nat Struct Biol 1998 Jul;5(7):561-7. PMID:9665169
    Data retrieval
  • Asymmetric unit, PDB entry: [header only] [complete with coordinates] (69 Kb) [Save to disk]
  • Biological Unit Coordinates (1say.pdb1.gz) 325 Kb
  • CSU: Contacts of Structural Units for 1SAY
  • Likely Quarternary Molecular Structure file(s) for 1SAY
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  • Classification of representative domains in scop (Structural Classification of Proteins)
        - Domain d1saya2, region A:1-135,A:304-361 [Jmol] [rasmolscript] [script source]
        - Domain d1saya1, region A:136-303 [Jmol] [rasmolscript] [script source]
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  • View one-letter amino acid or nucleotide sequence for each chain: [1say_A]
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