1CHP Toxin date Feb 15, 1995
authors E.A.Merritt, W.G.J.Hol
compound source
Molecule: Cholera Toxin B Pentamer
Chain: D, E, F, G, H
Synonym: Choleragen
Engineered: Yes
Mutation: G33d
Organism_scientific: Vibrio Cholerae
Strain: Ogawa 41 (Classical)
Expression_system: Escherichia Coli
symmetry Space Group: C 2
R_factor 0.178
crystal
cell
length a length b length c angle alpha angle beta angle gamma
102.600 66.610 99.710 90.00 131.57 90.00
method X-Ray Diffractionresolution 2.0 Å
ligand CL enzyme
similarity Belongs to the Enterotoxin_b family.
subunit The a subunit contains two chains, a1 and a2, linked by a disulfide bridge. The holotoxin (choleragen) consists of a pentameric ring of b subunits whose central pore is occupied by the a subunit.
gene ctxB (V. cholerae)
function It can bind five gm1 gangliosides. The b subunit pentameric ring directs the a subunit to its target by binding to the gm1 gangliosides present on the surface of the intestinal epithelial cells. It has no toxic activity by itself.
Gene
Ontology
ChainFunctionProcessComponent
H, F, D, G, E
  • pathogenesis
  • extracellular region
  • membrane
  • Primary referenceSurprising leads for a cholera toxin receptor-binding antagonist: crystallographic studies of CTB mutants., Merritt EA, Sarfaty S, Chang TT, Palmer LM, Jobling MG, Holmes RK, Hol WG, Structure 1995 Jun 15;3(6):561-70. PMID:8590017
    Data retrieval
  • Asymmetric unit, PDB entry: [header only] [complete with coordinates] (109 Kb) [Save to disk]
  • Biological Unit Coordinates (1chp.pdb1.gz) 88 Kb
  • CSU: Contacts of Structural Units for 1CHP
  • Likely Quarternary Molecular Structure file(s) for 1CHP
  • Structure Factors (293 Kb)
  • Retrieve 1CHP in mmCIF format [Save to disk]
  • View 1CHP in 3D
  • On Jmol, a nice Rasmol like molecule viewer. This is good for easiest viewing of basic structure.
  • On FirstGlance, an excellent tool for a guided tour on the structure components, by E. Martz.
  • On AstexViewer, from MSD-EBI (viewer documentation).
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  • STING, shows the sequence and maps the locations of sequence residues or residue ranges onto the 3D structure.
  • GRASS (Graphical Representation and Analysis of Structure Server)
  • Cartoon representation from PDB Cartoon
  • Ramachandran plot from PDBSum
  • Structure-derived information
  • Electron Density related parameters from EDS Electron Density Server, at Upsala
  • Dipole moment, from Dipole Server at Weizmann Institute
  • Crystal Contacts, from CryCo at Weizmann Institute
  • 3D motif for 1CHP, from MSDmotif at EBI
  • Genome occurence of 1CHP's fold from GeneCensus
  • Classification of representative domains in scop (Structural Classification of Proteins)
        - Domain d1chpd_, region D [Jmol] [rasmolscript] [script source]
        - Domain d1chpe_, region E [Jmol] [rasmolscript] [script source]
        - Domain d1chpf_, region F [Jmol] [rasmolscript] [script source]
        - Domain d1chpg_, region G [Jmol] [rasmolscript] [script source]
        - Domain d1chph_, region H [Jmol] [rasmolscript] [script source]
  • Fold representative 1chp from FSSP and Dali (Families of Structurally Similar Proteins)
  • Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH
  • Summaries and structural analyses of PDB data files from PDBSum
  • Identification of Protein Pockets & Cavities at CASTp
  • Sequence-derived information
  • View one-letter amino acid or nucleotide sequence for each chain: [1chp_G] [1chp_D] [1chp_E] [1chp_H] [1chp_F]
  • Other resources with information on 1CHP
  • InterPro: IPR001835
  • PDBREPORT (protein verification by WHAT_CHECK procedures)
  • MMDB (Entrez's Structure Database)
  • Movements, Movies and Images
  • Images from IMB Jena Image Library of Biological Macromolecules.

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